Corresponding Author's : Manisha Khatri

Co-Author's : Hemant Kardam, Vardan Wadhera, Vanshika Makol

Abstract :

Heat shock response is a cellular protection mechanism against unfavorable environmental conditions such as heat stress, resulting in inducible expression of heat shock proteins. The present study is designed to draw a comparative analysis between different strains of E.coli (DH5alpha, Rosetta, Codon+) under heat stress conditions, in terms of growth rates and protein expression. The strains were subjected to different temperature conditions (37°C, 45°C, 50°C, 55°C) for varied time periods (1hr, 3hr, 5hr, 12hr) and effect was studied in terms of OD600 values, which corresponds to bacterial population, and protein expression. Suppression of growth as indicated by lower OD600 and decreased colony numbers was observed at 55°C. Over-expression of some proteins, along with the synthesis of some new proteins was observed in all the strains at 55°C after incubation of 12h, which can be Hypothesised to be heat-shock proteins. Our results could add new outlook in order to understand the importance of heat shock response at such high temperatures in E.coli DH5alpha.

Keywords :

Heat stress, Protein expression, Growth rate, E.coli, Strains, Heat shock proteins

Article Details

Unique Paper ID: 47

Publication Volume & Issue: VOLUME 2 , ISSUE 2

Page(s): 62-65

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