Ascorbic acid is an essential micronutrient and a potent antioxidant for humans which performs pleiotropic functions related to the donation of electrons. Over the years, ascorbic acid is estimated by numerous methods like titration, and spectrophotometric, which are slightly more time-consuming, complex, and requires more chemicals. However, determination nowadays is becoming important because of its diversified roles in a simple, economical method and causing less harm to the environment. The Iodine clock method in this paper requires minimalistic chemicals, no spectrophotometer, and is easy to perform. It is a simple time-based, oxidation-reduction reaction in which Colorless reagents are mixed and following a short pause, the mixture turns blue–black in the presence of iodine. The chemical determination of ascorbic acid is based upon its highly reducing capacity by which oxidation of iodide ions to iodine takes place. This conversion is reported in time taken, which is generally a few seconds. This paper covers the estimation of ascorbic acid in a few organic samples by the iodine clock method and a comparative analysis with other conventional methods. Thus, the iodine clock method holds as a potential method for the estimation of ascorbic acid in the coming future.
Ascorbic acid, oxidation-reduction, iodine-clock, anti-oxidant, green analytical chemistry
Unique Paper ID: 68
Publication Volume & Issue: VOLUME 3 , ISSUE 4
Page(s): 32-35